ObjectiveTo understand the clinical distribution and antimicrobial resistance characteristics of clinically isolated carbapenemresistant Klebsiella pneumoniae (CRKP) and carbapenemresistant Escherichia coli （CREC）. MethodsClinically isolated Klebsiella pneumoniae and Escherichia coli from a hospital between January 2010 and December 2016 were analyzed retrospectively, isolation of CRKP and CREC were conducted statistical analysis. ResultsFrom 2010 to 2016, a total of 310 carbapenemresistant Enterobacteriaceae (CRE) isolates (268 were CRKP strains and 42 were CREC strains) were collected, isolation rate of CRKP increased from 1.33% in 2010 to 12.70% in 2016, showing an increasing trend year by year (χ2=123.73, P< 0.01); isolation rate of CREC was relatively stable, were all about 1.00% from 2010 to 2016. Most specimens were respiratory tract specimens (45.49%) and from intensive care unit (31.93%). Antimicrobial susceptibility testing showed that except resistance rates of CRKP and CREC were slightly lower to amikacin (80.60% and 38.10% respectively),  resistance rates of CRKP to most commonly used antimicrobial agents, such as third generation cephalosporins, fourth generation cephalosporins, βlactamase/lactamase inhibitors, and quinolones were all＞90%, resistance rates of CREC to above antimicrobial agents were all about 80%, resistance rate of nonCRE strains was lower than that of CRE strains (P<0.01). ConclusionDuring the past 7 years, detection rate of clinically isolated CRKP increased rapidly, were almost resistant to commonly used antimicrobial agents, which should be paid close attention.