ObjectiveTo explore the effect of meticulous nursing intervention and multidisciplinary team(MDT) collaboration on the compliance and correctness of wearing masks of tuberculosis(TB) patients going out for checkup. MethodsTB patients in the tuberculosis department of a hospital from October 2015 to December 2016 were investigated. OctoberDecember 2015 was pre intervention group（without intervention）, JanuaryDecember 2016 was postintervention group, meticulous nursing intervention and MDT collaboration were adopted, wearing status of medical surgical masks and awareness of TB prevention and control measures in TB patients going out for checkup before and after intervention were compared. Results799 TB patients were admitted in hospital, 160 cases were in pre intervention group and 639 in postintervention group. Before intervention, mask wearing rate and correct mask wearing rate were 50.63% and 86.42% respectively; after intervention, mask wearing rate and correct mask wearing rate were 95.31% and 98.52% respectively. Wearing rate and correct wearing rate of masks of TB patients at different seasons before and after intervention were compared, difference were both statistically significant (χ2=222.94, 38.512，respectively, both P=0.000）. After intervention, patients’ awareness of tuberculosis and prevention and control measures were both higher than before intervention, difference were both statistically significant (both P<0.001). ConclusionMeticulous nursing intervention and MDT collaboration can improve the wearing rate and correct wearing rate of masks in TB patients going out for checkup.