ObjectiveTo understand species, distribution, and antimicrobial resistance of common pathogens from patients with sepsis in intensive care unit（ICU），and provide reference for rational application of antimicrobial agents in sepsis patients. MethodsClinical data of sepsis patients who were admitted to the ICU of a hospital from January 2015 to June 2016 were collected retrospectively, pathogenic detection and antimicrobial susceptibility testing results of specimens were analyzed. ResultsA total of 175 sepsis patients were selected, 241 pathogens were isolated, 129 of which were multidrugresistant organisms(MDROs); 139 (57.68%) were gramnegative bacteria, 68 (28.22%) were grampositive bacteria, and 29 (12.03%) were fungi; the top 5 isolated strains were Escherichia coli (E. coli, 20.75%), Klebsiella pneumoniae (K. pneumoniae, 14.11%), Acinetobacter baumannii (A. baumannii, 13.28%), Candida albicans (C. albicans, 12.03%), and Pseudomonas aeruginosa (P. aeruginosa, 9.54%). The top five isolated MDROs were A. baumannii (29/32, 90.63%), Enterococcus faecium (16/20, 80.00%), K. pneumoniae (24/34, 70.59%), Staphylococcus spp. (14/21, 66.67%), and P. aeruginosa (14/23, 60.87%）. The isolation rates of extendedspectrum βlactamases (ESBLs)producing E. coli and K. pneumoniae were 68.00%(34/50) and 17.65%(6/34) respectively, E. coli had low resistance rates to carbapenems and βlactamase inhibitors, K. pneumoniae was only sensitive to tigecycline; A. baumannii was generally resistant to antimicrobial agents, P. aeruginosa was only sensitive to polymyxin; Enterococcus spp. and Staphylococcus spp. were sensitive to glycopeptide, linezolid, and tigecycline; C. albicans was generally sensitive to antifungal agents. ConclusionResistance rate of pathogens isolated from sepsis patients in ICU in this hospital is high, especially A. baumannii. Enzyme inhibitors, carbapenems, and glycopeptide are still effective antimicrobial agents for empirical antiinfection treatment.