Objective To investigate new medical personnels' sharp injuries and preventive measures. Methods Six hundred and sixty-eight new medical personnels who had been working in three affiliated hospitals of a university were investigated retrospectively through questionnaires. Results Among the new medical personnels investigated, 639 ( 95.66% ) persons injured 4 476 times, 90.87% of whom had two or more injuries. Most sharp injuries (63.43%) were caused by syringe needles, 32.77% of sharp injuries were caused by operating suture needle and knife blade, only 7.67% of injuries were reported. Conclusion Clinical new medical personnels have high incidence of the sharp injuries, the report of injuries was less common. Strengthen the education of comprehensive protective consciousness and safeguard procedures to reduce medical personnels' blood spread diseases is an important subject at present.