Objective To innovate the management of medical waste in medical institutions by means of information technology, and explore the effective management mode of medical waste. Methods A tertiary first-class hospital was selected, by means of information technology supporting management mode, the collection, transportation and temporary storage of medical waste were real-time monitored, plan-do-check-act(PDCA) management tool was used for continuous quality improvement in view of the problems found in the management. Change in awareness rate of medical waste-related knowledge, correct rate of medical waste disposal and occupational exposure rate rela-ted to medical waste before and after application of information management were statistically analyzed. Results Awareness on medical waste-related knowledge was surveyed, except the requirement of medical waste management system, the correct rates of other surveys after information management were all higher than before management, differences were all statistically significant (all P<0.01). In the daily inspection of medical waste disposal by ma-nagement department, except the standard temporary storage location of medical waste and protection measures for cleaners, the other inspection indicators after information management were all higher than before management, differences were all statistically significant (all P<0.01). After the implementation of information management, occupational exposure rate related to medical waste disposal was 0.005 case/person-year, which was lower than 0.011 case/person-year before the implementation, difference was statistically significant (P<0.05). Conclusion The effect of management mode of medical waste based on information technology is significant, which can reflect the effect of continuous improvement.