Objective To observe the effect of quality control circle (QCC) activity on reducing exceeding standard rate of microbial carrying on mobile phone surface of health care workers(HCWs). Methods QCC activity group was set up by department of healthcare-associated infection management and department of surgery, the theme of activity was "Reducing exceeding standard rate of microbial carrying on HCWs' mobile phone surface", causes of the problems were found and countermeasures were put forward, exceeding standard rate of microbial carrying on HCWs' mobile phone surface before and after implementing QCC activity was compared. Results The exceeding standard rate of microbial carrying on HCWs' mobile phone surface before and after implementing QCC activity were 36.82% and 13.15% respectively, exceeding standard rate of microbial carrying after QCC activity was lower than before QCC activity, difference was statistically significant (χ²=35.81,P<0.001). The goal achievement rate and the progress rate of exceeding standard rate of microbial carrying on mobile phone surface were 144.68% and 64.29% respectively. The circle ability increased from 60% before QCC activity to 85% after QCC activity. Conclusion QCC activity can reduce exceeding standard rate of microbial carrying on HCWs' mobile phone surface.