Objective To understand current status of job burnout and effort-reward imbalance(ERI) of full-time staff who engage in healthcare-associated infection(HAI)management, and provide scientific basis for improvement. Methods Based on stratified random sampling method, 50 full-time staff in 16 secondary and 5 tertiary hospitals in Wuhu City were selected as the research objects, Chinese Maslach burnout inventory (CMBI)and ERI questionnaire were used in the survey, scores of three dimensions of job burnout in different work modes were compared. Results 50 questionnaires were distributed and 46 were available (3 persons failed to reply in time and 1 questionnaire was unqualified), the effective recovery rate was 92.00%. Job burnout rate of full-time HAI management staff in Wuhu City was 91.30%, rates of moderate and severe burnout were 43.48%; 89.13% of full-time staff were in ERI state (ERI index>1), 69.57% were in overload working state. There were significant differences in the scores of emotional exhaustion among full-time staff with different educational background and years of engaging in HAI management (both P<0.05); there were significant differences in the scores of depersonalization among full-time staff with different marital status, educational background, hospital level and monthly income level (all P<0.05); there were significant differences in the scores of low sense of achievement among full-time staff in different levels of hospitals (P<0.05). The scores of emotional exhaustion and depersonalization of full-time staff in ERI and overload working state were both higher than those with effort-reward balance and non-overload working state (both P<0.05). Conclusion Job burnout and ERI of full-time HAI management staff in Wuhu City are serious, which should be concerned.