Acinetobacter baumannii (A. baumannii) is one of the common pathogens causing healthcare-associa-ted infection(HAI). The formation of A. baumannii biofilm can lead to its long-term presence in hospital environment, increase drug resistance and pathogenicity, and repeated occurrence of infection. Biofilm formation and regulation is a complex dynamic process, which is influenced by multiple factors and regulated by various mechanisms. The study on influencing factors and regulatory mechanisms of biofilm formation is conducive to finding new targets and searching new antimicrobial agents for the treatment of A. baumannii infection. In this paper, the progress of biofilm formation, influencing factors and regulatory mechanisms of A. baumannii are reviewed in order to provide methods and references for controlling its infection.