Objective To search biochemical marks for evaluating the prognosis of acquired immunodeficiency syndrome(AIDS) complicated with Talaromyces marneffei (T. marneffei) infection. Methods AIDS patients with T. marneffei infection in a hospital from January 2016 to December 2017 were selected, they were divided into death group and survival group according to the prognosis, laboratory biochemical marks such as white blood cell count (WBC), platelet count(PLT), hemoglobin(HGB), adenosine deaminase(ADA), creatine kinase isoenzyme(CK-MB), lactate dehydrogenase(LDH), aspartate aminotransferase(AST), total bilirubin(TBIL), and CD4⁺T cell count were performed univariate and multivariate analysis. Results A total of 140 AIDS patients with T. marneffei infection were included, 32 were in death group and 108 in survival group. Univariate analysis showed that there were significant differences in WBC, PLT, ADA, CK-MB, LDH, AST, and TBIL between patients in death group and survival group(all P<0.01). Multivariate logistic regression analysis showed that ADA (OR, 0.056[95%CI, 0.013-0.252]) and LDH (OR, 0.135[95%CI, 0.032-0.566]) had statistical significance on the outcome of AIDS complicated with T. marneffei infection. Conclusion Serum ADA and LDH levels are important markers for evaluating prognosis of AIDS patients with T. marneffei infection, and their elevation suggests poor prognosis.