Objective To understand the prevalence of infection caused by 7 common respiratory tract virus, and provide reference for clinical diagnosis and treatment. Methods Clinical data of patients with acute respiratory tract infection (ARTI) from March 2015 to February 2018 in Nanping First Hospital Affiliated to Fujian Medical University were retrospectively analyzed, antigens of 7 common respiratory virus were detected by direct immunofluorescence assay (DIA). Results A total of 5 874 ARTI patients were enrolled. The positive detection rate of virus was 19.58%(n=1 150). Detection rates in 2015-2017 were 11.48%, 21.71%, and 21.54% respectively. 1 131 patients (98.35%) were with single virus infection, 19 patients (1.65%) were with mixed infection. Among 7 detected common virus, respiratory syncytial virus (RSV) had the highest positive rate (11.29%). There was significant difference in the positive detection rate of ARTI virus among different age groups (P<0.05). Respiratory virus detection rate was the highest in~6 months group (26.37%), accounting for 39.83% of the positive patients. Positive rate of ARTI patients in different seasons was significantly different (P<0.05). Positive detection rate of respiratory virus was the highest in winter (25.01%), followed by autumn (23.23%). There was significant difference in the positive detection rate of virus among ARTI patients with different clinical diagnosis(P< 0.05), positive detection rate of virus in patients with bronchopneumonia was the highest (28.60%). Conclusion In the past three years, infection rate of common respiratory tract virus in ARTI patients increased year by year, RSV infection is most common among 7 respiratory tract virus infection. Respiratory tract virus infection mostly occur in autumn and winter, and children under the age of 1 year are the main infected population, bronchopneumonia is the main clinical manifestation.