Objective To understand the carrying status of carbapenemase gene and homology of carbapenem-resistant Klebsiella pneumoniae (CRKP) in a hospital, so as to provide laboratory basis for preventing and controlling transmission of CRKP clone strain. Methods 22 strains of CRKP (K1-K22) isolated from clinical departments in a hospital from January to December 2017 were collected, antimicrobial susceptibility testing results were re-checked by Kirby-Bauer disk diffusion and broth microdilution method, carbapenemase production was detected by modified Hodge test and Carba NP test, common carbapenemase genes of enzyme-producing strains were amplified and sequenced by polymerase chain reaction(PCR), homology analysis was performed by multilocus sequence typing (MLST) and enterobacterial repetitive intergenic consensus PCR(ERIC-PCR). Results Resistance rates of 22 CRKP to ertapenem, imipenem and meropenem were all 100%, resistance to other common clinical antimicrobial agents were also high; 13 strains of CRKP were positive for modified Hodge test and 14 were positive for Carba NP test. KPC-2 gene was carried by 14 enzyme-producing strains, NDM-1 gene was also carried by K12 strain. According to MLST method, CRKP strains were divided into ST11 (n=14), ST875 (n=6), ST1964(n=1), and ST571(n=1). According to ERIC-PCR method, CRKP strains were divided into type A (n=15), type B (n=6) and type C (n=1). K1-K6 strains with the same typing results were from intensive care unit (ICU), K7-K10 were from cerebrovascular surgery, and K15-K21 were from neonatology, corresponding patients had the same hospital stay, and they had been transferred (patients K2 and K8 were transferred from cerebrovascular surgery to ICU, K13 and K14 from ICU to departments of hematology and nephrology respectively). Conclusion Cloning epidemics of ST11 and ST875 CRKP existed in this hospital in 2017, it is necessary to strengthen the prevention and control measures of healthcare-associated infection.