Objective To analyze antimicrobial susceptibility and carrying status of drug resistance genes of carbapenem-resistant Klebsiella pneumoniae (CRKP) in a hospital. Methods CRKP isolated from a hospital between January 2017 and June 2018 were collected, antimicrobial susceptibility of strains was analyzed, polymerase chain reaction (PCR) was used to detect carrying status of drug resistance genes. Results A total of 57 strains of CRKP were collected, mainly from respiratory tract specimens, including sputum (n=34) and alveolar lavage fluid (n=11). The main source departments of CRKP were department of neurology (n=20, 35.09%), department of respiratory medicine (n=15, 26.32%), and department of critical medicine (n=9, 15.79%). CRKP was resistant to most antimicrobial agents, but resistance rates to some antimicrobial agents were relatively low, compound sulfamethoxazole had the lowest resistance rate (15.79%), followed by tegacycline (50.88%) and amikacin (57.89%). Two carbapenemase genes (KPC-2, NDM-1) and four extended-spectrum β-lactamases (ESBLs) genes (SHV, CTX-M-9, TEM, CTX-M-1) were detected. 57 strains of CRKP were all detected ESBLs genes, 39 (68.42%) of which were detected KPC-2 gene, and only 1 was detected NDM-1 gene. Conclusion Antimicrobial resistance of clinically isolated CRKP is severe, CRKP carries multiple drug resistance genes, the most common carbapenemase is KPC.