ICU水龙头及其周围污染情况的调查
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高晓东

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R181.3+2

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Contamination status of faucets and their surroundings in intensive care unit
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    摘要:

    目的 检测重症监护病房(ICU)中水龙头污染情况及污染范围,为水龙头周围的合理布局及降低水源性感染提供依据。方法 选取某三级综合医院ICU两处水龙头出水口及其周围环境进行棉拭子取样,以出水口为中心,每20~30 cm取一个样本,使用ATP检测技术、常规微生物检测以及二代测序技术(NGS)三种方式对标本进行检测,分析水龙头及其周围污染情况及飞溅范围。结果 在常规清洁的情况下,水龙头及其周围70 cm范围内污染严重。ATP检测合格率为60.0%,常规微生物检测合格率为73.3%。两个水龙头出水口棉拭子ATP检测结果分别为2 526、687 RLU/件,微生物检测显示菌落数均>105 CFU/cm2。在距离出水口70 cm的区域采样检测,菌落数仍然超标。NGS检测显示,从水龙头出水口到周围环境,均发现覆盖度较高的奥斯陆莫拉菌,与出水口细菌分布一致。结论 水龙头是病区内重要的污染源,应重视水龙头及其周围环境的清洁,1 m范围内,不应放置清洁物品以及与患者相关的物品。

    Abstract:

    Objective To detect status and scope of faucet contamination in intensive care unit(ICU), so as to provide basis for rational layout around the faucets and reducing water-borne infection. Methods Cotton-swab sampling method was used to take specimens from two faucet outlets and their surroundings in ICU of a tertiary general hospital, taking the outlet as the center, each specimen was taken every 20-30 cm, specimens were detected by ATP technique, conventional microbial detection and next-generation sequencing technique(NGS), contamination status and splash range around faucets and their surroundings were analyzed. Results In the case of conventional cleaning, faucets and their surrounding area within 70 cm was highly contaminated. The qualified rates of ATP detection and conventional microbial detection were 60.0% and 73.3% respectively. ATP detection results of two faucet outlets were 2 526 RLU/piece and 687 RLU/piece respectively, microbial detection showed that the number of colonies were both>105 CFU/cm2. In the area 70 cm away from the outlets, the number of bacterial colonies still exceeded the standard. The NGS detection showed that Moraxella osloensis with high coverage was found from the faucet outlets to their surroundings, which was consistent with the distribution of bacteria at the outlets. Conclusion Faucet is an important source of contamination in the ward, cleaning of faucet and its surroundings should be paid attention, clean articles and articles related to patients should not be placed within 1 meter.

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廖丹, 胡必杰, 史庆丰,等. ICU水龙头及其周围污染情况的调查[J]. 中国感染控制杂志,2019,18(6):566-570. DOI:10.12138/j. issn.1671-9638.20195342.
LIAO Dan, HU Bi-jie, SHI Qing-feng, et al. Contamination status of faucets and their surroundings in intensive care unit[J]. Chin J Infect Control, 2019,18(6):566-570. DOI:10.12138/j. issn.1671-9638.20195342.

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  • 收稿日期:2019-04-10
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  • 在线发布日期: 2019-06-28
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