Objective To evaluate efficacy of cleaning, disinfection and drying of new material medical mattress. Methods Referring to the Disinfection Technical Specifications (2002 version) and Hygienic Requirement for Medical Detergent (revision for approval), a test method for evaluating the efficacy of cleaning, disinfection and drying of medical mattress materials was established. Cleaning efficacy was determined by visual observation and residual protein detection method, disinfection and drying efficacy were determined by bacterial counting method and weighing method respectively. Results After the mattress carrier passed through a specific process, the qualified rates of cleaning efficacy were both 100% by visual observation method and residual protein detection method, mattress carrier after cleaning was not deformed, the contaminated mattress carrier was immersed in two disinfectants for 15 minutes, disinfection efficacy was qualified without deformation; water residue rate was 0.24% after hanging water mattress carrier for 1.5 hours. Conclusion The new material mattress can meet the requirements of overall cleaning, disinfection and drying, it opens up a new way for cleaning and disinfection of medical mattress, but on-site cleaning and disinfection efficacy, material washing resistance and cost-effectiveness need further research and verification.