Objective To check whether the status of performance operation of pre-vacuum team sterilizers is normal after displacement. Methods The temperature and pressure detector as well as chemical, physical, and biological monitoring methods were used to evaluate the operating status of sterilizers, the unqualified sterilizers were repaired and operation status of sterilizers after loading was monitored until all indicators shown as qualified. Results Among the four sterilizers that were moved, sterilizers No. 2, 3, and 4 were in good working condition, while sterilizer No. 1 had problems. The physical, biological monitoring, and lumen process challenge device (PCD) detection in sterilizer No. 1 before loading were qualified, the sterilizer fault was found by PCD (type 6) and B-D tests, temperature and pressure detector failed to pass the test. The potential causes of failures were examined by the engineers from the manufacturers of sterilizer as well as temperature and pressure detector. After three repairs, sterilization temperature and pressure of sterilizers were improved, sterilizer detection parameters were significantly improved and became qualified, the No. 1 sterilizer was finally adjusted to the optimum operating state. Conclusion In addition to routine monitoring, it is necessary to use temperature and pressure detector to measure the temperature, pressure, and time of sterilizer according to the Standard WS 310.3-2016. At the same time, it is also recommended to use the temperature and pressure detector as mandatory procedure after each new installation, displacement and overhaul of the sterilizer.