Objective To analyze the growth law, research hotspots and frontier of relevant literatures on healthcare-associated infection(HAI) training in China, and explore its development direction. Methods Based on China National Knowledge Infrastructure (CNKI) database, Excel table was employed to analyze the law of literature growth, and CiteSpace visualization software was introduced to identify the hotspots and frontier of domestic research on the training of HAI by means of co-occurrence network analysis on key words. Results A total of 1 267 articles were included in analysis, literature publication has gone through three stages:the initial stage (1991-2003), the growth stage (2004-2013) and the stable stage (2014 and beyond), the main publications were Chinese Journal of Nosocomiology, Journal of Traditional Chinese Medicine Management and Chinese Journal of Infection Control. A total of 131 nodes and 387 connecting lines were identified by key word co-occurrence network. The hotspots of identified research were "compliance", "hand hygiene", "operating room", "healthcare-associated infection", "health care worker", "pre-job training" and "cleaners", with the centrality values ≥ 0.30. These research hotspots present certain evolution rules with "knowledge training" and "primary medical institutions" as the recent frontier. Conclusion The development of HAI training research in China is closely related to the development of national HAI management. The training system for non-professional personnel needs to be improved in the future, and the research on training system and effect evaluation for full-time personnel needs to be strengthened.