Objective To understand the basic situation and sources of pressure of full-time staff in healthcare-associated infection(HAI) management in a city of Henan Province. Methods A stratified random sampling method was used to classify hospitals into secondary and tertiary hospitals according to the level of hospitals, all were public hospitals, secondary and tertiary hospitals were numbered, target hospitals were investigated by random sampling, the general situation, profession, full-time HAI management personnels matching, willing to continue to work in HAI management and sources of pressure of full-time staff in HAI management were investigated. Results A total of 44 full-time staff in HAI management in 17 secondary and tertiary general hospitals were investigated. Full-time personnels mainly received college education(43.18%); professional titles were mainly intermediate titles(63.64%); profession was mainly nursing(81.82%); service years in HAI management were 5 to 10 years(47.73%); the highest proportion of age was 40-50 years old (34.09%). Only 4 full-time personnels matching met the requirements(23.53%). Thirty full-time staff (68.18%) were willing to change their jobs, 40-50 years old were the most(86.67%), followed by 30-39 years old (78.57%). Among the main sources of pressure of full-time staff in hospitals of different levels, the top five were:high work pressure (95.45%), extensive management (81.82%), insufficient attention of hospital leaders (72.73%), few full-time staff (61.36%) and difficult promotion of professional titles (50.00%). Conclusion The irrational structure of the full-time staff in HAI management, high work pressure, extensive management and insufficient leadership attention are the main sources of pressure of full-time staff in HAI management in this city.