Objective To investigate the staffing of health care workers(HCWs) in blood purification institutions in Suzhou City, and provide baseline data for improving the staffing of HCWs in blood purification institutions in Suzhou. Methods A cross-sectional survey was conducted in 39 non-independent medical institutions that provided medical services of blood purification technology in a city in August 2017, self-designed blood purification questionnaire for HCWs was used. Data were analyzed statistically by Excel 2013 and SPSS 17.0. Results Of the 39 blood purification institutions, the number of doctors in 37 institutions was up to the standard, qualified rate was 94.87%; the number of nurses in 7 blood purification institutions was up to the standard, qualified rate was 17.95%. The number of nursing staff in 10 administrative regions of the city is insufficient, among which county-level city G has the highest proportion (0.47), district F has the lowest proportion (0.33). The proportion of HCWs with intermediate and higher titles was the highest (92.31%) in district F and the lowest (35.42%) in district B(P<0.01), the tertiary medical institutions was higher than that in the secondary, primary and non-graded medical institutions, with a statistically significant difference (P<0.05). Conclusion There is a shortage of nursing staff in the staffing of HCWs in blood purification institutions in this city, and constituent of professional titles of HCWs in some blood purification institutions is irrational.