Objective To analyze disease causes, distribution and drug resistance of pathogens causing infective endophthalmitis(IE). Methods Clinical data of patients with IE in a hospital from January 2016 to December 2018 were retrospectively analyzed. Results 90 cases of IE were mainly caused by ocular trauma (56.7%), 20 (22.2%) were caused by eye surgery, 19 (95.0%) of which were caused by cataract surgery combined with artificial lens implantation. Among 52 strains of pathogens causing IE, Gram-positive bacteria accounted for 50.0% (26 strains), mainly Staphylococcus epidermidis (13 strains), Gram-negative bacteria accounted for 30.8% (16 strains), mainly Pseudomonas aeruginosa (6 strains) and Klebsiella pneumoniae (4 strains). Susceptibility rates of Staphylococcus epidermidis to vancomycin, linezolid, rifampicin and tigecycline were all 100.0%, while susceptibility rates to penicillin and oxacillin were both low (< 50%). Conclusion IE is mainly caused by ocular trauma and eye surgery, especially cataract surgery, the main pathogens are Staphylococcus epidermidis, Pseudomonas aeruginosa and Klebsiella pneumoniae.