Objective To analyze the incidence and clinical characteristics of infectious endophthalmitis after different types of intraocular surgery/manipulation, provide reference for prevention and control of healthcare-associa-ted infection(HAI)in ophthalmology hospital. Methods Patients who underwent intraocular surgery/manipulation in a hospital from January 2017 to December 2019 were analyzed retrospectively, occurrence of infectious endophthalmitis was analyzed. Results A total of 36 107 patients (mean age 50.78±19.96 years) undergoing intraocular surgery/manipulation were collected; the main types of intraocular surgery were cataract surgery (52.10%), implantable contact lens(ICL) surgery for high myopia (19.67%) and vitrectomy (17.58%). A total of 20 patients developed infectious endophthalmitis, the overall incidence was 0.06%, of which 14 cases were postoperative acute endophthalmitis, 6 cases were delayed-onset endophthalmitis; among different surgical types, ICL surgery had the lowest incidence of infectious endophthalmitis (0.01%), and glaucoma surgery had the highest incidence of infectious endophthalmitis (0.12%). A total of 12 strains of pathogens were isolated from 20 patients with infectious endophthalmitis, including 8 strains of Gram-positive bacteria, 3 strains of Gram-negative bacteria and 1 strain of fungus; 12 patients (60.00%) were treated with pars-plana vitrectomy, and 18 patients were cured, the cure rate was 90.00%. Conclusion Incidence of infectious endophthalmitis is varied in different types of intraocular surgery/manipulation, prevention of infectious endophthalmitis should be conducted according to different types of operation.