Objective To analyze the epidemiological and spatial distribution characteristics of adenovirus infection in Wuming District of Nanning City, so as to provide scientific basis for the prevention of adenovirus outbreak. Methods A unified epidemiological questionnaires on individual case were used to conduct one-to-one investigation, geographic information system (GIS) technology was adopted to explore the spatial distribution and clustering of the epidemic, spatial epidemiological distribution characteristics of the epidemic was analyzed by kernel density analysis and Moran's I index. Results A total of 228 children were infected with adenovirus in this outbreak, and none of them were seriously ill or died. The age of the infected patients was mainly 5-9 years old, and the onset time was mainly from July 28 to August 4, 2019. The major clinical manifestations were fever, cough, pharyngeal congestion and tonsillar enlargement. Moran's I Z=3.76 (P<0.05), suggesting that the spatial distribution of patients with adenovirus infection has a strong spatial clustering, and kernel density analysis showed that infected children in this epidemic were distributed in the whole region. Conclusion There is a strong population mobility during the summer vacation, spread of the epidemic in a large scale should be alerted. Parents should strengthen their own safety awareness and develop good hygiene habit when taking their children out to play in public places. The use of spatial analysis can comprehensively and intuitively show the clustering region of epidemic diseases, and provide scientific reference for the prevention and control of the epidemic.