Objective To analyze carrying status of drug-resistant genes of clinically isolated imipenem-resistant Acinetobacter baumannii (IRAB) in a hospital, provide laboratory basis for prevention and control as well as gui-dance for clinical treatment of HAI. Methods Polymerase chain reaction (PCR) was used to detect carrying status of carbapenem-resistant genes (blaIMP, blaKPC, blaNDM-1, blaOXA-23, blaOXA-50, blaOXA-51, and blaOXA-58), aminoglycoside-resistant genes (ant[3"]-Ⅰ, aac[6']-Ⅰ, armA) and disinfectant-resistant gene qacE△1 of 26 clinically isolated IRAB strains in this hospital. Results 26 strains of IRAB all carried carbapenem-resistant genes OXA-23 and OXA-51,detection rate was 100%,other carbapenem-resistant genes were not found. The detected rates of aminoglycoside-resistant genes ant (3")-Ⅰ, aac (6')-Ⅰand armA were all 96.15%, the carrying rate of disinfectant-resistant gene qacE△1 was 65.38%. 92.31% of clinically isolated IRAB strains were from sputum of patients. Conclusion The majority of clinically isolated IRAB strains in this hospital carry the same carbapenem-resistant gene and aminoglycoside-resistant gene, it is necessary to strengthen the prevention and control measures of HAI.