Objective To understand occupational exposure of health care workers(HCWs) in designated medical institutions for coronavirus disease 2019(COVID-19) treatment in Hunan Province. Methods According to the documents issued by Health Commission of Hunan Province, occupational exposure of HCWs in fever clinics and isolation wards at 365 medical institutions in Hunan Province was reported every Tuesday and Friday, data were collec-ted by Hunan Provincial Healthcare-associated Infection Quality Control Center, HCWs' occupational exposure related to COVID-19 treatment were analyzed. Results From January 27 to March 3, 2020, a total of 29 HCWs with occupational exposure related to COVID-19 treatment were reported, 5(17.24%) were males and 24 (82.76%) were females; the average age was (32.39±6.95) years old; 22 were nurses and 4 were doctors, 24 of whom were aid HCWs in fever clinics and isolation wards. The main exposure location was COVID-19 isolation wards (n=26). High-, medium-, and low-risk exposure were 12 (41.38%), 14 (48.28%), and 3 cases (10.34%) respectively; the main type of exposure was sharp injury (n=18, 62.07%); the most common manipulations were venous blood collection(n=5, 17.24%) and arterial blood gas analysis (n=4, 13.79%); the main exposure sites were hands or fingers (n=18, 62.07%); among HCWs who had occupational exposure related to COVID-19, 27(93.10%)had second-level protection when exposed. 19 occupational exposure sources were patients, 7 of whom were with confirmed COVID-19. 29 HCWs with occupational exposure were all reported to department of HAI control or health care department of the hospital in time, by March 17, they all exceeded more than 14-day isolation observation period, 1 occupational exposure HCW developed COVID-19. Conclusion During COVID-19 epidemic period, HCWs' personal protection consciousness has been greatly improved, but new occupational exposure risk links can result in post-exposure infection, occurrence of occupational exposure can be effectively reduced by improving manipulation level of HCWs under the second-level protection, providing high-quality protective equipment and complying with the manipulation regulations.