Objective To analyze the epidemiological characteristics of a suspected outbreak of carbapenem-resis-tant Klebsiella pneumoniae (CRKP) healthcare-associated infection(HAI), provide evidence for prevention and control of HAI. Methods Field epidemiological and environmental hygiene detection methods were adopted to investigate patients with CRKP during hospitalization in pediatric intensive care unit(PICU) of a children's hospital from June 19 to July 10, 2019, the causes were analyzed and appropriate prevention and control measures were taken. Results Epidemiological investigation showed that 6 patients were isolated CRKP, and 5 of whom had HAI (3 cases of lower respiratory tract infection, 1 case of bacteremia, 1 case of catheter-related bloodstream infection), 1 case was colonization. A total of 177 environmental hygiene specimens were collected, 2 specimens (hanging tower and floor towel) were isolated CRKP, the remaining specimens were not found CRKP. After taking control mea-sures, no similar new cases occurred in the PICU until the end of July 2019. Conclusion The suspected outbreak of CRKP HAI may be transmitted through the surface of PICU environment and hands of health care workers, strengthening hand hygiene of HCWs and taking strict disinfection measures can effectively control the further pre-valence of CRKP.