Objective To understand 2019-nCoV contamination on hand-touched buttons of alcohol-based hand rub in the isolation ward area of a regional coronavirus disease 2019(COVID-19) treatment hospital, and provide evidence for guiding disinfection in isolation ward area. Methods Hand-touched buttons of alcohol-based hand rub in 5 regions of the isolation ward area (confirmed ward, ward corridor, first buffer room, second buffer room, clean zone) were sampled, and contamination in specimens were detected with reverse transcription polymerase chain reaction (RT-PCR). Results A total of 76 button specimens were collected, 2019-nCoV contamination rate in isolation ward area was 10.53%, contamination rates of buttons in confirmed ward and ward corridor were 14.29% and 12.50% respectively, contamination rates in the first buffer zone, the second buffer zone, and clean zone were all 0%. Conclusion Hand-touched buttons of alcohol-based hand rub in this isolation ward area are seriously contaminated by 2019-nCoV, cleaning and disinfection needs to be strengthened, it is suggested that buttons in isolation ward area should be pressed by the back of the hand, if medical institution is conditional, inductive liquid outlet and hand-washing sensor monitor should be used.