Objective To understand the implementation of environmental disinfection in medical institutions(MIs) in China, provide scientific basis for the implementation and revision of Regulation of disinfection technique in healthcare settings (WS/T 367-2012). Methods By multi-stage stratified sampling method, 168 MIs in 6 pro-vinces were selected, monitoring results of disinfection mode, frequency and efficacy of air and object surface in general wards and high risk departments were surveyed, filled and reported on internet by full-time staff of infection management in MIs. Results A total of 163 MIs participated in the survey, 2 of which didn't open general wards. 75 (46.58%) MIs disinfected air in general wards, 42 (56.00%) disinfected with ultraviolet light, 31 (41.33%) only performed terminal disinfection or disinfected when necessary, 98 (60.87%) regularly disinfected object surface in general wards, 94 (95.92%) disinfected once or twice a day; 73 (45.34%) MIs monitored the effect of environmental disinfection in general wards, 47 (64.38%) of which monitored at least once per quarter. 141 (86.50%) MIs disinfected air in high risk departments, 122 (86.52%) of which disinfected twice a day; 163 (100%) MIs disinfected object surface of high risk departments, 134 (82.21%) of which disinfected at least twice a day; 161 (98.77%) MIs regularly monitored the effect of environmental disinfection in high risk departments, 111 (68.94%) of which monitored once a quarter. Conclusion The issuing of Regulation of disinfection technique in healthcare settings (WS/T 367-2012) has promoted the environmental cleaning and disinfection in all levels of MIs, but the phenomenon of excessive and insufficient disinfection and monitoring of general wards exists in MIs, which needs further improvement.