Objective To explore hospitalization expense and length of hospital stay in patients with healthcare-associated infection(HAI) due to multidrug-resistant organisms(MDROs) in the intensive care unit(ICU). Methods 1 378 patients who were admitted to ICU in a tertiary hospital from October 2017 to December 2018 were recruited as the study objects, 167 of whom had MDROs infection (MDROs group), 1 211 patients without MDROs infection were in non-MDROs group. MDROs group and non-MDROs group were matched in a 1:1 ratio by propensity score matching(PSM), hospitalization expense and length of hospital stay of matched patients were analyzed statistically. Results A total of 166 pairs were successfully matched by using PSM, the successful matching rate was 99.40%, the median length of hospital stay in MDROs group and non-MDROs group were 34 and 16 days respectively, median difference was 18 days, difference between two groups was statistically significant (P<0.001); the total hospitalization expense was 220 205.83 and 93 380.81 Yuan respectively, difference was 126 825.02 Yuan, difference between two groups was statistically significant (P<0.001). Except the expense of image diagnosis, expense of medical service, treatment manipulation, nursing, experimental diagnosis, clinical diagnosis, western medicine and antimicrobial agents in MDROs group were all higher than non-MDROs group (all P<0.05), difference in expense of western medicine between two groups was the largest (63 828.46 Yuan). Conclusion MDROs healthcare-associated infection(HAI) in ICU patients will increase the length of hospital stay and economic burden of patients, preventive and control measures should be taken.