Objective To evaluate the disinfection effect of a new hypochlorous acid disinfectant on fiberbronchoscopes. Methods 60 used fiberbronchoscopes were strictly cleaned in accordance with the operation procedures, then fiberbronchoscopes were performed machine washing and disinfection with hypochlorous acid disinfectant for 3 and 5 minute respectively. ATP bioluminescence assay and membrane filtration method were used to monitor and evaluate the disinfection effect on the outer and inner surface of fiberbronchoscopes,result was compared with before cleaning and enzyme washing. Results The median value of relative light unit(RLU) on the outer surface of 30 used fiberbronchoscopes before cleaning was 911.5, qualified rate was 16.7%; after enzyme washing but before machine washing, the median value of RLU on the outer surface of fiberbronchoscopes was 234.0, qualified rate was 43.3%. After each group of 30 fiberbronchoscopes were immersed in disinfectant for 5 and 3 minutes respectively, median values of RLU of the outer surface of fiberbronchoscopes were 25.0 and 23.5 respectively, qualified rates were both 100.0%. Concentration of hypochlorous acid disinfectant could maintain at effective concentration (≥ 80 mg/L) after continuous use for 7 days. Conclusion The new hypochlorous acid disinfectant can achieve good disinfection effect on fiberbronchoscopes for 3-minute contact time, and improve work efficiency with good safety and stability.