Objective To understand contamination of SARS-CoV-2 in isolation wards, and provide evidence for precise monitoring on healthcare-associated infection(HAI). Methods Object surface specimens were taken from clean area, potentially contaminated area and contaminated area in the SARS-CoV-2 isolation wards in The First Hospital of Changsha before disinfection, referring to pharyngeal swab test method, specimens were performed SARS-CoV-2 nucleic acid test, test results were analyzed statistically. Results A total of 191 specimens of various object surface in isolation wards were collected, 9 of which were positive for SARS-CoV-2 test, positive rate was 4.71%. Positive specimens were all from contaminated areas of SARS-CoV-2 isolation wards, ventilator panel, probe and outer surface of B-ultrasound machine, foot pedal of medical waste bin, patient's quilt cover, bedside lamp switch, hands of health care workers(HCWs), bedside table, toilet door handle, patient's mobile phone were detected one positive specimen respectively. Specimens of object surface of potentially contaminated area and clean area was not found SARS-CoV-2. In contaminated areas, positive rate of SARS-CoV-2 nucleic acid test of surroun-ding of patients with cough was higher than that of patients without cough (10.81% vs 1.43%, P<0.05); there was no statistical difference among positive rates of patients' personal belongings, patients' daily necessities, medical facilities and equipment, as well as hands of HCWs and patients (P>0.05). Conclusion Contaminated area in isolation wards is contaminated seriously by SARS-CoV-2, especially surrounding of patients with cough. Strict disinfection and isolation measures should be taken to effectively reduce the HAI risk of COVID-19.