Objective To construct and evaluate the quality assessment index system of healthcare-associated infection(HAI) in the hospital performance assessment system. Methods HAI quality assessment index system was preliminary designed, HAI quality assessment index in hospital performance assessment system was determined through two rounds of Delphi expert consultation and analytic hierarchy process. Results A set of HAI quality assessment system was constructed, including 2 first-class indexes, 9 second-class indexes and 38 third-class indexes, positive coefficient of two rounds of Delphi expert consultation was 100%, authority coefficient was 0.826 and 0.852 respectively; coordination coefficient of the second round was relatively low, and expert opinions tended to be consistent. The top five indexes of combination weight of second-class indexes were infection-related indicator (0.204), infection safety (0.155), infection assessment (0.110), disinfection and sterilization (0.109), and hand hygiene (0.103). The top five indexes of combination weight of third-class indexes were antimicrobial use in infected patients (0.052), preoperative antimicrobial use (0.048), implementation of hand hygiene indication (0.045), grasp of infection knowledge (0.044), and HAI rate (0.042). The reliability and validity test showed that Cronbach's α coefficient of the index system was 0.788, r values of retest reliability and structural validity were both >5, difference were both significant (both P<0.05). Conclusion HAI quality assessment index system constructed in this study can evaluate HAI management performance fairly and objectively.