Objective To analyze the clinical features of nocardiosis, so as to provide experience for the diagnosis and treatment of nocardiosis. Methods Clinical data of 13 patients with nocardiosis in Sichuan Provincial People's Hospital from January 2016 to November 2020 were analyzed retrospectively. Results Among 13 patients, there were 5 cases of pulmonary infection, 2 cases of central nervous system infection, 4 cases of skin infection, 1 case of disseminated infection and 1 case of suppurative arthritis. 12 patients had underlying diseases, mainly bronchiectasis, autoimmune diseases, diabetes mellitus, and hypertension. All patients had increased leukocytes and C-reactive protein (CRP), only 3 patients detected procalcitonin (PCT), and PCT all decreased. Pathogenic examination results were Nocardia brasiliensis. Nine patients received compound sulfamethoxazole alone or in combination, 3 patients received levofloxacin alone, and 1 patient with disseminated infection received carbapenems combined with linezolid. Brain abscess and skin infection are treated surgically, operation or debridement. All 12 patients improved, and only 1 patient with suppurative meningitis aggravated. Conclusion Nocardiosis mainly includes pulmonary infection, central nervous system infection, skin infection and disseminated infection, patients with bronchiectasis or autoimmune diseases are more likely to be infected.