高黏液表型与基因型预测肺炎克雷伯菌毒力的比较
作者:
作者单位:

1.东莞市第一人民医院呼吸内科, 广东 东莞 523000;2.广州医科大学附属第一医院呼吸内科, 广东 广州 510000

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通讯作者:

卓超  E-mail: chaosheep@sina.com

中图分类号:

R446.5

基金项目:

广州市科技重点项目(201607020044)


Comparison of hypermucoviscous phenotype and genotype in predicting virulence of Klebsiella pneumoniae
Author:
Affiliation:

1.Department of Respiratory Diseases, Dongguan People's Hospital, Dongguan 523000, China;2.Department of Respiratory Diseases, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, China

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    摘要:

    目的 评价高黏液表型、毒力基因预测高毒力肺炎克雷伯菌(hvKp)的应用价值。 方法 收集2016年11月—2018年4月广东省4所医院分离的肺炎克雷伯菌,通过拉丝试验判断菌株黏液表型,检测毒力基因和血清型情况,采用蜡螟模型计算菌株的半数致死率(LD50),比较不同高黏液(hm)表型和毒力基因型菌株LD50结果 共收集194株肺炎克雷伯菌,拉丝试验阳性菌株占40.2%(78株)。蜡螟试验结果显示,仅毒力基因iucA、iroB、fimH阳性菌株LD50低于相应的阴性菌株(P < 0.05)。iucA+iroB+fimH+hm全阳性组(n=58)、iucA阳性+iroB阳性+fimH阳性+hm阴性组(n=23)、iucA+iroB+fimH+hm全阴性组(n=28)3组LD50分别为(5.39±0.71)、(5.44±0.62)、(5.83±0.64)log10 CFU/mL,前两组比较差异无统计学意义(P=0.786),但前两组分别与第3组比较差异均有统计学意义(P < 0.05)。在高黏液表型菌株中血清型K1、K2、K57检出率高于非高黏液表型菌株,差异均有统计学意义(均P < 0.001)。 结论 仅依据高黏液表型预测hvKp准确性较低,与高黏液表型相比,毒力基因iucA+、iroB+、fimH+预测hvKp更准确。

    Abstract:

    Objective To evaluate the application value of hypermucoviscous phenotype and virulence gene in predicting hypervirulent Klebsiella pneumoniae (hvKp). Methods Klebsiella pneumoniae isolated from four hospitals in Guangdong Province from November 2016 to April 2018 were collected, mucoviscous phenotypes were determined through string test, virulence genes and serotypes were detected, 50% lethal rate (LD50) of strains was calculated by Galleria mellonella model, LD50 of strains of different hypermucoviscous phenotypes and virulence genes were compared. Results A total of 194 strains of Klebsiella pneumoniae were collected, 40.2% (n=78) of which were positive for string test. Galleria mellonella test results showed that LD50 of only iucA, iroB, and fimH positive strains was lower than that of corresponding negative strains(P < 0.05). LD50 of strain group of all positive for iucA+iroB+fimH+hm (n=58), iucA positive+iroB positive+fimH positive+hm negative (n=23) and all negative for iucA+iroB+fimH+hm (n=28) were (5.39±0.71), (5.44±0.62), and (5.83±0.64) lg10CFU/mL respectively, there was no significant difference between the first two groups (P=0.786), but there was significant difference between the first two groups and the third group (P < 0.05), positive rates of serotypes K1, K2 and K57 in hypermucoviscous phenotype strains were significantly higher than those in non-hypermucoviscous phenotype strains, difference were all significant (all P < 0.001). Conclusion Accuracy of the prediction of hvKp based only on hypermucoviscous phenotype is low, compared with hypermucoviscous phenotype, virulence genes iucA+, iroB+ and fimH + are more accurate to prediction hvKp.

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引用本文

马明葱,李晓雨,卓超.高黏液表型与基因型预测肺炎克雷伯菌毒力的比较[J]. 中国感染控制杂志,2021,(11):1003-1007. DOI:10.12138/j. issn.1671-9638.20211269.
Ming-cong MA, Xiao-yu LI, Chao ZHUO. Comparison of hypermucoviscous phenotype and genotype in predicting virulence of Klebsiella pneumoniae[J]. Chin J Infect Control, 2021,(11):1003-1007. DOI:10.12138/j. issn.1671-9638.20211269.

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  • 收稿日期:2021-03-24
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  • 在线发布日期: 2024-04-26
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