Acinetobacter baumannii is one of the common pathogens causing healthcare-associated infection, the emergence of multidrug-resistant organisms, even pandrug-resistant organisms, poses a great threat to the world public health security. In recent years, a number of experimental studies have proved that bacteria can regulate the expression of relevant antimicrobial resistance genes by generating specific non-coding RNAs (ncRNAs) or changing their conformation under the pressure of antimicrobial agents, and play an important role in regulating antimicrobial resistance of bacteria. This review summarizes the research on regulatory role of regulatory ncRNAs on antimicro-bial resistance of Acinetobacter baumannii and ncRNAs as drug targets, in order to provide help to deal with the problem of antimicrobial resistance of Acinetobacter baumannii.