Objective To analyze the risk factors for multidrug-resistant Escherichia coli (MDR-EC) infection in children aged 0-14 years, and explore the application value of bundle management in the prevention and control of infection. Methods Patients with infection in a hospital from June 1, 2016 to September 30, 2019 were selected as the research objects. The observation group was from June 1, 2016 to December 31, 2017, bundle management group was from January 1, 2018 to September 30, 2019. Clinical data of patients in observation group were analyzed, risk factors for MDR-EC infection were explored, bundle management scheme was adjusted according to the results, MDR-EC infection between two groups of patients was compared. Results A total of 343 patients in observation group and 328 patients in bundle management group were investigated. In observation group, 70 patients were with MDR-EC infection, the independent risk factors for MDR-EC infection in patients in observation group were non-ICU hospitalization time ≥ 14 days, ICU hospitalization time ≥ 7 days, respiratory tract infection, mecha-nical ventilation, mechanical ventilation ≥ 7 days, combined use of antimicrobial agents, and antimicrobial use time ≥ 14 days (all P<0.05). Compliance rate of hand hygiene in patients in bundle management group was higher than that in observation group (95.99% vs 94.39%). The proportion of MDR-EC infection in infection cases was lower than that in observation group (14.02% vs 20.41%), differences were both significant (both P<0.05). The mechanical ventilation time, central venous catheterization time, antimicrobial use time, hospitalization time and hospitalization expenses of bundle management group were all lower than those of observation group, differences were all statistically significant (all P<0.05). Conclusion MDR-EC infection in children is affected by many factors, bundle management can reduce the occurrence of infection.