Objective To evaluate the effect of bundle intervention on reducing catheter-associated urinary tract infection (CAUTI). Methods From January to June 2017, the baseline survey was conducted on targeted monitoring of patients with urinary catheterization in a hospital (pre-intervention period); from July to December, combined with plan-do-check-act (PDCA) tool, the main causes were find out and bundle intervention measures were formulated (transitional period); from January to June in 2018, intervention measures were implemented (post-intervention period), difference in urinary catheter utilization rate, average urinary catheterization days, and incidence of CAUTI per 1 000 catheter-days before and after intervention were compared. Results After intervention, compliance rate of hand hygiene, implementation rate of prevention and control measures, as well as awareness rate of prevention and control measures were 81.94%, 95.37% and 97.98% respectively, which were higher than 64.66%, 66.31% and 65.02% before the intervention, implementation rate of daily assessment reached 96.15% after intervention. Incidence of CAUTI per 1 000 urinary catheter-days decreased from 1.71‰ before intervention to 1.22‰ after intervention, catheter utilization rate dropped from 10.02% before intervention to 7.69% after intervention, incidence of CAUTI per 1 000 urinary catheter-days in internal medical wards dropped from 3.73‰ before intervention to 1.56‰ after intervention, differences between these indexes before and after intervention were all statistically significant (all P < 0.05). Conclusion CAUTI target monitoring combined with PDCA tool can identify the weak links of HAI prevention and control, provide evidence for formulating specific prevention and control measures, and implement bundle intervention measures to reduce the incidence of CAUTI effectively.