Hepatitis C is a serious public health issue threatening people's health. At present, there is no vaccine to prevent hepatitis C virus (HCV), but combination treatment of direct antiviral drug can cure more than 95% of chronic hepatitis C. Strengthening the screening of HCV-infected person and conducting effective treatment as early as possible is an effective measure to eliminate the source of infection and block the transmission of HCV. This guideline is revised and updated on the basis of 2012 edition of Chinese guideline for the prevention and control of healthcare-associated infection of hepatitis C, which mainly includes the epidemiology and current situation of healthcare-associated infection of hepatitis C, laboratory detection methods and clinical significance of HCV infection, as well as prevention and control measures of healthcare-associated infection, so as to provide help for the prevention of hepatitis C healthcare-associated infection.