Objective To explore the effect of multi-disciplinary team (MDT) management in the prevention and control of healthcare-aasociated infection(HAI)caused by multidrug-resistant organisms (MDROs) in children. Methods Children who were isolated MDROs in a tertiary first-class children's hospital between January 1, 2017 and December 31, 2018 were selected as research objects. Routine MDRO HAI prevention and control measures were adopted from January 1 to December 31, 2017 before intervention, and MDT management mode was implemented from January 1 to December 31, 2018. The compliance rate of prevention and control measures, MDRO isolation rate, case incidence of HAI, department distribution and constituent ratio of MDRO infection were compared between two groups of children before and after implementation. Results After the implementation of MDT ma-nagement, compliance rates of various prevention and control measures increased significantly, including clinicians' compliance to give isolation order, medical record folder and patient list pasted with contact isolation sign, isolation sign hanging at the head of bed, alcohol-based hand rub placed at beside, compliance rate and accuracy rate of hand hygiene, proper disposal of medical waste, exclusive use of reusable medical devices, medical staff awareness of MDRO-infected children, correct formulation of disinfectant, inform relevant departments of transferred patient, as well as cleaning and disinfection of environmental object surface, differences were all statistically significant (all P < 0.05). The total isolation rate of MDROs decreased from 22.09% to 16.50%, isolation rates of carbapenem-resis-tant Klebsiella pneumoniae (CRKP), carbapenem-resistant Escherichia coli (CREC), methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant Acinetobacter baumannii (CRAB), and carbapenem-resistant Pseudomonas aeruginosa (CRPA) decreased from 19.25%, 10.38%, 27.42%, 36.39% and 15.37% to 15. 29%, 6.01%, 23.64%, 21.99% and 11.39% respectively, differences were all statistically significant (all P < 0.05). Vancomycin-resistant Enterococcus (VRE) were not found before and after the implementation of MDT management; case incidence of MDRO HAI decreased from 0.19% before implementation to 0.14% after implementation of MDT management, difference was statistically significant (χ²=4.800, P=0.028). Conclusion The implementation of MDT management can effectively improve the implementation rate of MDRO HAI prevention and control measures, and effectively reduce the occurrence of MDRO HAI in children.