Objective To explore the establishment and effect of healthcare-associated infection (HAI) monitoring team of clinicians and nurses based on bottom management model of three-level network in HAI management. Methods The combination of department recommendation and self-recommendation was adopted to select clinicians and nurses as monitoring staff, through defining responsibility, building systematic training, quantifying perfor-mance evaluation standards, monthly special allowance of 200 Yuan, title promotion priority and other incentive treatment, management of monitoring staff team was strengthened, HAI management monitoring indexes before and after the establishment of HAI monitoring team from 2014 to 2018 was compared, effectiveness was assessed. Results A total of 54 HAI control clinicians and 64 nurses were set up in the whole hospital. With the deepening and refinement of management, the scores of monitoring medical staff has improved significantly, incidence of HAI decreased from 2.41% before setting up to 1.17% after setting up, missing reporting rate of HAI decreased from 20.67% to 8.40%, isolation rate of multidrug-resistant organisms decreased from 0.27% to 0.12%, hand hygiene compliance increased from 38.70% to 58.25%, specimen detection rate prior to antimicrobial use increased from 41.18% to 74.08%, incidence of ventilator-associated pneumonia and catheter-related urinary tract infection decreased from 10.47‰ and 3.11‰ to 2.42‰ and 1.13‰ respectively, differences were all significant (all P < 0.05). Conclusion Based on bottom management model of three-level network in HAI management, implementation of innovative management mode can effectively improve the enthusiasm of clinicians and nurses in HAI monitoring, which play a positive role in promoting HAI management and improving the quality of HAI management in the whole hospital.