Objective To investigate the setting status of hand hygiene (HH) facilities in a tertiary first-class comprehensive teaching hospital based on the concept of point-of-care (POC). Methods A cross-sectional survey was conducted on the type, number, location, facility completeness of HH facilities and consumption of alcohol-based hand rub used by health care workers in each diagnosis and treatment section of this hospital. Results 102 sections were surveyed, there were 1 165 diagnosis and treatment rooms, setting rate of HH facilities was 96.48%, completeness rate of hand washing sink was 91.37%, there was significant difference in completeness rate in diffe-rent sections (P < 0.01). 80.53% of alcohol-based hand rub setting complied with the POC concept, 59.51% of alcohol-based hand rub setting in the general wards complied with the POC concept, consumption of hand disinfec-tant was relatively higher in departments with high conformity to POC concept for the setting of alcohol-based hand rub. Conclusion The setting and improvement of HH facilities should strengthen the POC concept to improve HH compliance and prevent the occurrence of healthcare-associated infection.