Objective To explore an effective rinsing method for reusing ophthalmic surgical instruments under the special condition of "Health Express" without running water cleaning equipment. Methods The study was divided into two stages to explore the effect of different rinsing methods on the removal effect and cleaning quality of multi-enzyme detergents for surgical instruments. The cataract microsurgical instruments without contamination and si-mulated clinical surgical contamination were selected respectively. Group A, B, C, D, F and G were trial groups, surgical instruments were rinsed with non-running water under the condition of simulated "Health Express", rinsing methods of group A, B and C were "2 L/4 L/6 L water combined with brushing", group D was with "no rinsing", group F adopted the same rinsing method as group B, group G adding "0.5 L water combined with rinsing" on the basis of group B. Group E and H served as the control group, using running water to rinse surgical instruments in accordance with the guidelines. Evaluation of cleaning effect: protein residue detector was used to detect indirectly the residue of multi-enzyme detergent, and ATP bioluminescence detector was used to detect the cleaning quality of surgical instruments. Results The residual amount of multi-enzyme detergent in group B was (1.90±0.23) μg per 20 surgical instruments, difference between multi-enzyme detergent residue in group B and control group ([3.45±0.46] μg per 20 surgical instruments) was not significant (P=0.053). ATP biofluorescence detection result in group G was (9.46±1.50) RLU per surgical instrument, difference between ATP biofluorescence detection result in group G and control group ([11.60±1.44] RLU per instrument) was not significant (P=0.339). Conclusion Combined with the results of two-stage experiments, group G rinsing method is recommended for rinsing ophthalmic surgical instruments under the special condition of "Health Express" without running water cleaning equipment.