首页 > 过刊浏览>2022年第卷第1期 >55-61. DOI:10.12138/j.issn.1671-9638.20221773
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猩红热患儿A族链球菌emm基因分型及地域与毒力基因分布相关研究
作者:
作者单位:

1.深圳市儿童医院呼吸科, 广东 深圳 518038;2.首都医科大学附属北京朝阳医院儿科, 北京 100043;3.华中科技大学同济医学院附属武汉儿童医院急诊科, 湖北 武汉 430000;4.国家儿童医学中心 首都医科大学附属北京儿童医院 北京市儿科研究所微生物研究室, 北京 100045;5.上海复旦大学附属儿童医院细菌实验室, 上海 201102

作者简介:

梁云梅为共同第一作者。

通讯作者:

郑跃杰  E-mail：yuejiez@sina.com
杨永弘  E-mail：yyh628628@sina.com

中图分类号:

R181.3;R515.1

基金项目:

深圳市医学重点学科建设经费(SZXK032);广东省医学科研基金(A2021437);广东省高水平临床重点专科(深圳市配套建设经费)(SZGSP012)


Relationship among emm gene typing, regional and virulence gene distribution of group A Streptococcus strains isolated from children with scarlet fever
Author:
Affiliation:

1.Department of Respiratory Diseases, Shenzhen Children's Hospital, Shenzhen 518038, China;2.Department of Pediatrics, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100043, China;3.Department of Emergency, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science&Technology, Wuhan 430000, China;4.Microbiology Laboratory, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China;5.Department of Bacteria Laboratory, Shanghai Children's Hospital Affiliated to Fudan University, Shanghai 201102, China

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    摘要:

    目的 探讨猩红热儿童分离来源的A族链球菌（GAS）emm分型、地域与毒力基因分布的相关性。 方法 采集北京和上海地区猩红热患儿标本进行GAS分离鉴定，应用聚合酶链式反应（PCR）方法检测emm基因型以及13种毒力基因（speA、speB、speC、speF、speG、speH、speI、speJ、speK、speL、speM、ssa和smeZ）携带情况，分析GAS菌株毒力基因分布与地域、emm基因型的关系。 结果 114株GAS分离株中，emm1.0型60株，emm12.0型54株；52株分离自北京（emm1.0型31株，emm12.0型21株），62株分离自上海（emm1.0型29株，emm12.0型33株）。speA、speC、speG、speH、speI、speJ、speK、ssa和smeZ基因携带率分别为52.6%、76.3%、80.7%、43.9%、55.3%、57.0%、75.4%、97.4%、93.0%，speB和speF携带率均为98.2%，未检出speL、speM基因。北京地区emm1.0型与emm12.0 GAS菌株speA、speH、speI和speJ基因携带率比较，上海地区emm1.0型与emm12.0 GAS菌株speA、speH、speI、speJ和speK基因携带率比较，差异均有统计学意义（均P < 0.05）。emm1.0型GAS菌株speC、speH、speJ和speK基因携带率北京与上海两地区比较，差异均有统计学意义（均P < 0.05），emm12.0型GAS菌株speC、speH、speI、speJ和speK基因北京与上海两地区比较，差异均有统计学意义（均P < 0.05）。 结论 GAS菌株毒力基因的分布状态因emm基因分型及地域不同存在差异。

    Abstract:

    Objective To investigate the relationship between emm typing, regional and virulence gene distribution of group A Streptococcus (GAS) isolated from children with scarlet fever. Methods Specimens from children with scarlet fever in Beijing and Shanghai were collected for GAS isolation and identification, emm genotype and carrying status of 13 kinds of virulence genes (speA, speB, speC, speF, speG, speH, speI, speJ, speK, speL, speM, ssa and smeZ) were detected by polymerase chain reaction (PCR), relationship between distribution of virulence gene and region as well as emm genotype of GAS strains was analyzed. Results Among 114 GAS strains, 60 were emm1.0 and 54 were emm12.0; 52 strains were isolated from Beijing(31 strains were emm1.0 and 21 were emm12.0), and 62 strains were from Shanghai(29 were emm1.0 and 33 were emm12.0). The carrying rates of speA, speC, speG, speH, speI, speJ, speK, ssa and smeZ were 52.6%, 76.3%, 80.7%, 43.9%, 55.3%, 57.0%, 75.4%, 97.4%, and 93.0% respectively, carrying rates of speB and speF were both 98.2%, speL and speM genes were not found. There were significant difference in carrying rates of speA, speH, speI and speJ genes of emm1.0 and emm12.0 GAS strains in Beijing as well as speA, speH, speI, speJ and speK genes of emm1.0 and emm12.0 GAS in Shanghai (all P < 0.05). Carrying rates of speC, speH, speJ and speK of emm1.0 GAS strain, as well as carrying rates of speC, speH, speI, speJ and speK of emm12.0 strain between Beijing and Shanghai were all significant (all P < 0.05). Conclusion Distribution of virulence genes of GAS strains is different with emm genotyping and region.

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引用本文

禹定乐,梁云梅,卢清华,等.猩红热患儿A族链球菌emm基因分型及地域与毒力基因分布相关研究[J]. 中国感染控制杂志,2022,(1):55-61. DOI:10.12138/j. issn.1671-9638.20221773.
Ding-le YU, Yun-mei LIANG, Qing-hua LU, et al. Relationship among emm gene typing, regional and virulence gene distribution of group A Streptococcus strains isolated from children with scarlet fever[J]. Chin J Infect Control, 2022,(1):55-61. DOI:10.12138/j. issn.1671-9638.20221773.

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  • 收稿日期:2021-08-12
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  • 在线发布日期: 2024-04-28
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