People infected with human immunodeficiency virus (HIV) have different degrees of immune deficiency, which may lead to the lower vaccination effectiveness of SARS-CoV-2 vaccines than the normal population. HIV-infected person should be the priority group for booster shots of SARS-CoV-2 vaccines. However, at present, there are no reports of HIV-infected people receiving adenovirus vector vaccines for sequential booster immunization at home and abroad after receiving SARS-CoV-2 inactivated vaccines. Therefore, this paper reports a case of HIV-infected person whose HIV was well suppressed and CD4⁺ T cell count was normal after receiving antiretroviral therapy (ART). After receiving two doses of SARS-CoV-2 inactivated vaccines (Beijing Biological Research Institute of Biological Products, Sinopharm China Co., Ltd.), sequential booster immunization was performed through vaccinating adenovirus vector vaccine (CanSino Biologics Inc., Ltd.), which was safe and highly immunogenic, and can guide the population to vaccinate against SARS-CoV-2.