Objective To understand the current situation and problems of cleaning and disinfection of water-perfused esophageal manometry catheter in China. Methods Cleaning, disinfection and sterilization of water-perfused esophageal manometry catheter in 31 hospitals in China was performed questionnaire survey. Results All 31 hospitals used high-resolution water-perfused esophageal manometry catheter, 22.58% used traditional manometry catheters at the same time. 87.10% of hospitals used 1-2 high-resolution water-perfused esophageal manometry catheter, 54.84% of hospitals used catheter in ≤10 patients per month. 58.06% of hospitals had specially assigned persons to clean and disinfect water-perfused esophageal manometry catheter, nurses in 93.55% of hospitals participated in catheter cleaning and disinfection. The cleaning and disinfection process was mainly "initial washing-enzyme wa-shing-rinsing-disinfection-final washing" (96.77%), most hospitals injected and washed the internal channels of catheter one by one in each step (93.55%), the initial washing time was mainly 3-5 minutes (48.39%); 96.77% of hospitalschose the disinfection method of complete immersion of chemical disinfectant, disinfectant was mainly orthophtha-laldehyde (38.71%), disinfection time was mainly 5-10 minutes (74.19%). Only 29.03% of hospitals sterilized water-perfused esophageal manometry catheter, mainly "one sterilization only used for once" (88.89%) and immersion in glutaraldehyde for 10 hours (66.67%). The sterilized water-perfused esophageal manometry catheter was mainly hung vertically in the gastroscope storage room (51.61%), and 38.71% of hospitals disinfected water-perfused esophageal manometry catheter to be used on that day again. Conclusion There are some deficiencies and differences in the cleaning, disinfection and sterilization of water-perfused esophageal manometry catheter in 31 hospitals, it is suggested that the relevant national standards for cleaning and disinfection of water-perfused esophageal manometry catheter should be formulated as soon as possible, so as to standardize the cleaning and disinfection process of water-perfused esophageal manometry catheter.