Objective To understand antimicrobial resistance and virulence gene carrying of clinically isolated carbapenem-resistant Klebsiella pneumoniae (CRKP), so as to provide basis for clinical prevention and treatment. Methods 36 strains of clinically isolated CRKP in a hospital from March 2020 to March 2021 were collected, antimicrobial susceptibility identification of strains were performed, antimicrobial resistance genes, capsular serotype genes and virulence genes were detected by polymerase chain reaction (PCR) amplification, sequence typing (ST) was performed by multilocus sequence typing, serotyping and molecular evolutionary tree analysis were performed based on wzi sequencing results. Results 36 CRKP strains were all detected bla_KPC gene, but bla_IMP, bla_VIM, and bla_OXA-48 genes were not found. String test was negative, four common capsule serotypes K1, K2, K5, K20, and K57 were not found, detection rates of rmpA2, wcaG, ybtS, aerobactin, iutA, iroN, ycf, mrkD, mrkA, silS, uge, Plvpk, fimH, and wzi genes of 36 CRKP strains were 100%; TerW was 86.11%; fimA and magA were both negative. MLST analysis showed that 36 strains were all ST11, only 32 strains were successfully sequenced wzi gene. wzi typing results were K14. K64 96.88% (31/32) and K24 3.12% (1/32). Molecular evolutionary tree analysis based on sequencing results showed that 31 of 32 strains were 100% homologous, and 1 strain was 99% homologous with Zhejiang strain KP18069. Conclusion The main mechanism of resistance of CRKP to carbapenems in this hospital is bla_KPC gene, the dominant ST is ST11, and wzi type is mainly K14.K64, which carries a large number of virulence genes. Molecular evolutionary tree indicates that there is homologous infection, and it is suspected that there is import transmission. Prevention and control measures should be taken in time to prevent the transmission of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) in the hospital.