Objective To analyze the characteristics of norovirus (NoV) healthcare-associated infection (HAI) in children. Methods Based on early active screening analysis, detection result of NoV in hospitalized children in Guangzhou Women and Children's Medical Center from January 2019 to March 2021 was analyzed by prospective study, NoV HAI in children was analyzed, genotyping was performed with reverse transcription-polymerase chain reaction (RT-PCR). Results Among 2 659 cases of HAI, there were 421 cases of gastrointestinal tract infection, 188 cases of gastrointestinal tract infection (44.7%) were conducted NoV detection; 96 cases (51.1%) were positive for NoV. There were 24 cases of NoV HAI among hospital staff, including 13 cleaners and 11 health care workers. Among children with NoV HAI, 66 were males and 30 were females; the average age was (37.6±41.10) months, and 81.3% were under 5 years old; autumn and winter had high incidence; 95.8% were transmitted by contact. 17 cases (17.7%) were asymptomatic carriers, and 79 cases of symptomatic infection were mainly change in stool shape (69.6%) and vomiting (44.3%). 20.8% of cases were related to HAI cluster epidemic. 70 cases (72.9%) of children with NoV HAI got better and 26 cases (27.1%) were cured. 90.9% of 44 NoV positive specimens were type G Ⅱ. Conclusion NoV has a great impact on children's gastrointestinal HAI and can cause cluster epidemic. Early active screening on NoV can detect disease early, isolation and preventive measures can be taken as early as possible according to the seasonal epidemic characteristic, common transmission modes, and asymptomatic infected person.