One bacterial strain isolated from human infection wound secretion was identified and performed antimicrobial susceptibility testing. Wound secretion specimen of the patient was collected, blood agar plate and McConkey agar plate were used for isolation and culture. Bacterial strain was identified as Bordetella spp. by VITEK 2 Compact automatic microbial identification system (confidence 97%), Bordetella trematum (B. trematum) was identified by VITEK MS automatic rapid microbial mass spectrometry detection system and 16S rRNA analysis (confidence 99.9%). Antimicrobial susceptibility testing showed that B. trematum was resistant to cefazolin, ceftazidime, cefepime, cefatriaxone, gentamicin, tobramycin, levofloxacin, and compound sulfamethoxazole, it is mediate resistant to piperacillin/tazobactam, imipenem and meropenem, while susceptible to amikacin. This case is the first report on the isolation of B. trematum from clinical specimens in China, which provides a reference for guiding the rapid and accurate identification of B. trematum infection and clinical rational antimicrobial use.