The main transmission mode of healthcare-associated infection (HAI) is contact transmission, whether the cleaning and disinfection of high-touch surfaces is qualified is very important for the prevention and control of HAI. This paper mainly reviewed the research progress of current situation and harm, cleaning and disinfection methods, and effect evaluation of contamination of high-touch surfaces in high risk areas of hospitals at domestic and abroad, so as to provide suggestions and references for HAI managers and future scientific research on cleaning and disinfection of high-touch surfaces in hospitals.