Objective To investigate the distribution and antimicrobial resistance of pathogens of candidemia in a hospital, and explore the application efficacy of standardized intervention measures for healthcare-associated infection (HAI). Methods Patients who were hospitalized in a hospital in Huai'an City from January 2015 to December 2019 were select as the control group, and those from January 2020 to December 2022 were as the intervention group. Standardized HAI intervention measures were implemented. Clinical data of patients with candidemia were analyzed, including bacterial distribution, antimicrobial resistance, and HAI incidence. Results There were a total of 507 974 hospitalized patients in the control group and 392 616 in the intervention group. Over eight years, there were 181 cases of candidemia, 76 of which were HAI-associated candidemia. Among the 181 Candida strains, the proportions of Candida albicans, Candida glabrata, Candida dubliniensis, Candida tropicalis and Candida parapsilosis were 38.12%, 21.55%, 14.92%, 14.37% and 9.39%, respectively. Difference in the clinical distribution of HAI-associated Candida was statistically significant (P < 0.05). Candida tropicalis had high antimicrobial resistance (P < 0.05). In the control group, there were 117 cases of candidemia, with an incidence of 0.23 ‰; and 58 HAI cases, with a HAI incidence of 0.11 ‰. In the intervention group, there were 64 cases of candidemia, with an incidence of 0.11 ‰; and 18 HAI cases, with a HAI incidence of 0.05‰. The incidences of candidemia and HAI were both statistically significant between the two groups (both P < 0.05). The mortalities of candidemia and HAI-associated candidemia were 6.08% and 7.89%, respectively. Conclusion The clinical distribution of HAI-associated candidemia is sporadic, and antimicrobial resistance of strains is unspecific. The implementation of standardized HAI intervention measures can reduce HAI-associated candidemia.