Objective To explore the effect of improving cleaning and disinfection methods on the prevention, control and disinfection of carbapenem-resistant Enterobacterales (CRE) in burn plastic surgery ward. Methods 297 patients who admitted to the department of burn plastic surgery in a hospital from February 1 to August 31, 2021 were selected as the control group, and 210 patients who admitted to the hospital from September 1, 2021 to February 28, 2022 after cleaning and disinfection methods improved were selected as the intervention group. Detection rate of CRE from patients, incidence of healthcare-associated infection (HAI) with CRE, and detection rate of environmental CRE before and after intervention were statistically analyzed and compared. Results The incidence of HAI and detection rate of CRE from patients in the intervention group were 0.95% and 0, respectively, lower than 4.04% and 2.02% in the control group (both P < 0.05). Compared to the control group, qualified rates of detection of air and surface microbiology, adenosine triphosphate (ATP) bioflorescence and fluorescence labeling in the intervention group were all higher(χ²=5.52, 13.08, 6.66, and 15.01, respectively, all P < 0.05). Conclusion Improving cleaning and disinfection method can reduce the incidence of HAI and the detection rate of CRE in burn wards, improve the surface cleanliness of environmental objects, as well as the effectiveness of HAI prevention and control.