Objective To explore the sealing effect of pre-filled catheter irrigator on central venous catheter. Methods Patients treated with hemodialysis through jugular vein catheterization in the blood purification center of a tertiary first-class hospital from January 2020 to June 2021 were selected. Patients who met the inclusion criteria were divided into the observation group and the control group according to the random number table method. Patients in the observation group used the pre-filled catheter irrigator to seal the central venous catheter, and those in the control group used heparin sodium sealing fluid to seal the catheter. The occurrence of catheter-related blood-stream infection (CRBSI), patency and sealing cost of catheter, duration of catheter irrigation and occurrence of needle-stick injury during catheter sealing were analyzed. Results A total of 85 patients were included, including 42 in the observation group and 43 in the control group. Incidence of CRBSI in the observation group (0.79 ‰, 1/1 260) was lower than that in the control group (5.43 ‰, 7/1 290), and the catheter irrigation time in the observation group ([6.44±1.11] seconds) was shorter than that in the control group ([220.04±5.98] seconds). Diffe-rences between two groups were statistically significant (both P < 0.05). There were 3 cases of catheter blockage in the observation group and 4 cases in the control group, without statistically significant difference between two groups (P=0.728). There was no needle-stick injury in the observation group when the catheter was sealed, however, 5 cases of needle-stick injury occurred in the control group, difference between two groups was statistically significant (P < 0.05). The average costs of catheter sealing for patients in the observation group and control group were 11.40 Yuan and 15.14 Yuan, respectively. Conclusion Pre-filled catheter irrigator can effectively reduce the incidence of CRBSI in patients undergoing hemodialysis through jugular vein catheterization, save costs, reduce needle-stick injury, and improve work efficiency.